Hiroshi Ishikita, PhD (Dr. rer. nat.)

Group Leader (Assistant Professor)
International Young Scientists Career Development Organization
Graduate School of Medicine
Kyoto University, Japan

Contact Address
Building E, Graduate School of Medicine, Kyoto University
Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan

EDUCATION & ACADEMIC EXPERIENCE

4/2008-12/2008
Assistant Professor
Institute of Molecular and Cellular Biosciences
The University of Tokyo, Japan

12/2006-3/2008
Postdoctoral Research Associate (Prof. Arieh Warshel's group)
Department of Chemistry
University of Southern California, USA

12/2005-11/2006
Postdoctoral Research Associate (Prof. Sharon Hammes-Schiffer's group)
Department of Chemistry
The Pennsylvania State University, USA

11/2005
PhD (Dr. rer. nat.) 
(thesis: summa cum laude, disputation: summa cum laude, and overall: summa cum laude)
Freie Universität Berlin 
Adviser: Prof. Ernst-Walter Knapp
(2nd Adviser: Prof. Wolfram Saenger)

4/2004-12/2005
Wissenschaftlicher Mitarbeiter  (Research Associate)
Kristallographie, Institut für Chemie und Biochemie
Freie Universität Berlin (Project: Sfb498, A5)

10/2000-3/2004
DAAD Stipendiat
Freie Universität Berlin (Free University of Berlin), Germany
(Prof. Ernst-Walter Knapp's group)

6/2000-9/2000
DAAD, Deutscher Akademischer Austausch Dienst, Stipendiat (DAAD scholarship holder)
Goethe-Institut Bremen, Germany

3/2000
Master (Chemistry and Biotechnology, Engineering)
The University of Tokyo
Adviser: Prof. Teruyuki Nagamune

3/1998
Bachelor  (Chemistry and Biotechnology, Engineering)
The University of Tokyo, Japan

Scholarship & Fellowship
DAAD Stipendium (scholarship) (2000-2004)
JSPS fellowship for research abroad (2007-)

PUBLICATIONS

total 29 (1st: 26, others: 3)

1. Hiroshi Ishikita
   J. Biol. Chem. 283 (2008) 30618-30623
   "Light-induced hydrogen bonding pattern and driving force of electron transfer in AppA BLUF domain photoreceptor"
   Journal
   
   
2. Hiroshi Ishikita
   Biochemistry 47 (2008) 4394-4402
   "Redox potential difference between Desulfovibrio vulgaris and Clostridium beijerinckii flavodoxins"
   Journal Pubmed
   
   
3. Hiroshi Ishikita and Arieh Warshel
   Angew. Chem. 120 (2008) 709-712
   Angew. Chem. Int. Ed. Engl. 47 (2008) 697-700
   "Predicting drug resistant mutations of HIV protease"
   Journal Pubmed
   
   
4. Sharon Hammes-Schiffer, Elizabeth Hatcher, Hiroshi Ishikita, Jonathan Skone, and Alexander V. Soudackov
   Coord. Chem. Rev. 252 (2008) 384-394
   ”Theoretical studies of proton-coupled electron transfer: models and concepts relevant to bioenergetics”
   
   
5. Frank Müh, Mohamed El-Amine Madjet, Julia Adolphs, Ayjamal, Abdurahman, Björn Rabenstein, Hiroshi Ishikita, Ernst-Walter Knapp, and Thomas Renger
   Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 16862-16867 [Communicated by R. A. Marcus]
   ”a-Helices direct excitation energy flow in the Fenna-Matthews-Olson protein”
   Journal
   
   
6. Hiroshi Ishikita, Artur Galstyan, and Ernst-Walter Knapp
   Biochim. Biophys. Acta 1767 (Bioenergetics) (2007) 1300-1309
   ”Electron transfer of the non-heme iron complex in bacterial photosynthetic reaction center”
   Journal
   
   
7. Hiroshi Ishikita
   J. Biol. Chem. 282 (2007) 25240-25246
   ”Contributions of protein environment to redox potentials of quinones in flavodoxins from Clostridium beijerinckii”
   Journal PubMed
   
   
8. Hiroshi Ishikita
   FEBS Lett. 581 (2007) 4293-4297
   ”Modulation of the protein environment in the hydrophilic pore of the ammonia transporter protein AmtB upon the GlnK protein binding”
   Journal PubMed
   
   
9. Hiroshi Ishikita, Alexander V. Soudackov and Sharon Hammes-Schiffer
   J. Am. Chem. Soc. 129 (2007)  11146-11152
   Buffer-assisted proton-coupled electron transfer in a model rhenium-tyrosine complex"
   Journal PubMed
   
   
10. Irina Karygina, Dietmar Stehlik, Ingelog Wyndhamn, Art van der Est, Hiroshi Ishikita, Ernst-Walter Knapp, Barath Jaganathan, Rufat Agalarov, and John H. Golbeck
    Biochemistry 46 (2007) 10804-10816
    ”Contributions of the distant protein environment to the redox potentials of the A₁ phylloquinone and the F_X iron-sulfur cluster in photosystem I”
    Journal PubMed
    
    
11. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Am. Chem. Soc.129 (2007) 1210-1215
    ”Protonation states of ammonia/ammonium in the hydrophobic pore of ammonia transporter protein AmtB”
    Journal PubMed
    
    
12. Hiroshi Ishikita, Bernhard Loll, Jacek Biesiadka, Jan Kern, Klaus-Dieter Irrgang, Athina Zouni, Wolfram Saenger and Ernst-Walter Knapp
    Biochim. Biophys. Acta 1767 (Bioenergetics) (2007) 79-87 
    "Function of two b-carotenes near the D1 and D2 proteins in photosystem II dimers"
    Journal PubMed
    
    
13. Hiroshi Ishikita and Ernst-Walter Knapp
    FEBS Lett. 580 (2006) 4567-4570
    "Electrostatic role of the non-heme iron complex in bacterial photosynthetic reaction center"
    Journal PubMed
    
    
14. Hiroshi Ishikita, Wolfram Saenger, Jacek Biesiadka, Bernhard Loll, and Ernst-Walter Knapp
    Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 9855-9860 [Edited by H. Gray]
    "How photosynthetic reaction centers control oxidation power in chlorophyll pairs P680, P700 and P870"
    Journal PubMed
    
    
15. Hiroshi Ishikita and Ernst-Walter Knapp
    Biophys. J.  90 (2006) 3886-3896
    "Function of redox-active tyrosine in photosystem II"
    Journal PubMed
    
    
16. Hiroshi Ishikita, Jacek Biesiadka, Bernhard Loll, Wolfram Saenger, and Ernst-Walter Knapp
    Angew. Chem. 118 (2006) 1998-1999
    Angew. Chem. Int. Ed. Engl. 45 (2006) 1964-1965
    "Cationic state of accessory chlorophyll and electron transfer through pheophytin to plastoquinone in photosystem II"
    Journal PubMed
    
    
17. Hiroshi Ishikita, Wolfram Saenger, Bernhard Loll, Jacek Biesiadka, and Ernst-Walter Knapp
    Biochemistry 45 (2006) 2063-2071
    "Energetics of a possible proton exit pathway for water oxidation in Photosystem II"
    Journal PubMed
    
    
18. Hiroshi Ishikita, Dietmar Stehlik, John H. Golbeck and Ernst-Walter Knapp
    Biophys. J. 90 (2006) 1081-1089
    "Electrostatic influence of PsaC protein binding to the PsaA/PsaB heterodimer in Photosystem I"
    Journal PubMed
    
    
19. Hiroshi Ishikita and Ernst-Walter Knapp
    Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 16215-16220 [Edited by H. Gray]
    "Induced conformational changes upon Cd²⁺ binding at photosynthetic reaction centers"
    Journal PubMed
    
    
20. Hiroshi Ishikita and Ernst-Walter Knapp
    Biochemistry 44 (2005) 14772-14784
    "Oxidation of the non-heme iron complex in photosystem II"
    Journal PubMed
    
    
21. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Am. Chem. Soc. 127 (2005) 14714-14720
    "Control of quinone redox potentials in photosystem II: electron transfer and photoprotection"
    Journal PubMed
    
    

22. Hiroshi Ishikita and Ernst-Walter Knapp
    FEBS Lett. 579 (2005) 3190-3194
    "Redox potential of cytochrome c550 in the cyanobacterium Thermosynechococcus elongatus"
    Journal PubMed
    

23. Hiroshi Ishikita, Bernhard Loll, Jacek Biesiadka, Wolfram Saenger and Ernst-Walter Knapp
    Biochemistry 44 (2005) 4118-4124
    "Redox potentials of chlorophylls in the photosystem II reaction center"
    Journal PubMed
    

24. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Biol. Chem. 280 (2005) 12446-12450
    "Energetics of proton transfer pathways in reaction centers from Rhodobacter sphaeroides. The Glu-H173 activated mutants"
    Journal PubMed
    

25. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Am. Chem. Soc. 127 (2005) 1963-1968
    "Redox potentials of chlorophylls and b-carotene in the antenna complexes of photosystem II"
    Journal PubMed

26. Hiroshi Ishikita, Bernhard Loll, Jacek Biesiadka, Artur Galstyan, Wolfram Saenger and Ernst-Walter Knapp
    FEBS Lett. 579 (2005) 712-716
    "Tuning electron transfer by ester-group of chlorophylls in bacterial photosynthetic reaction center"
    Journal PubMed

27. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Am. Chem. Soc. 126 (2004)  8059-8064
    "Hydrogen bond between Ser-L223 and Q_B^-: protonation of Asp-L213 in the reaction center of Rhodobacter sphaeroides"
    Journal PubMed

28. Hiroshi Ishikita and Ernst-Walter Knapp
    J. Biol. Chem. 278 (2003)  52002-52011
    "Redox potential of quinones in both electron transfer branches of photosystem I"
    Journal PubMed
    

29. Hiroshi Ishikita, Giulia Morra, and Ernst-Walter Knapp
    Biochemistry 42 (2003) 3882-3892
    "Redox potential of quinones in photosynthetic reaction centers from Rhodobacter sphaeroides: dependence on protonation of Glu-L212 and Asp-L213"
    Journal PubMed

PRESENTATION

1. Ernst-Walter Knapp and Hiroshi Ishikita
   "Characterizing electron transfer in photosystem II"
   15th IUPAB & 5th EBSA International Biophysics Congress, July 27-August 1, Montpellier, France (2005) [Poster]
   
   
2. Hiroshi Ishikita and Ernst-Walter Knapp
   "Redox potentials of quinones controlled by hydrogen bonding pattern in photosystem II"
   13th International Congress of Photosynthesis (29/8-3/9/2004, Montreal, Canada)
    29-September 3 (2004) [Talk+Poster]
   
   
3. Hiroshi Ishikita and Ernst-Walter Knapp
   "Redox potential of quinones in both electron transfer branches of Photosystem I"
   XIth International Congress of Quantum Chemistry 2003, Bonn, Germany, July 20-26 (2003) [Poster]
   
   
4. Hiroshi Ishikita and Ernst-Walter Knapp
   "How do H-bonds Shift Redox Potentials of Cofactors in Proteins: Examples from Photosynthetic Reaction Centers"
   XVth International Conference on Horizons in Hydrogen Bond Research, Berlin, Germany, September 16-23 (2003) [Talk+Poster]