Matrix-technology

Research areas

The focus of our activities is directed at combinatorial libraries of nucleic acids. Functional nucleic acids can be identified from these libraries through in vitro evolution. We could identify mirror image nucleic acids (spiegelmers) from combinatorial libraries that bind with high affinity and specificity to a given target and display an extraordinary stability.

The objective of our recent work is to develop a matrix-technology that serves as a new platform for the analysis of gene function. We prepare screens of nucleic acids and proteins to assay for the interaction with other molecules. The screen allows the fast assignment of interacting proteins and the rapid identification of novel drug candidates.

Selected publications

Klußmann, S.; Nolte, A.; Bald, R.; Erdmann, V.A.; Fürste, J.P.: Mirror-image RNA that binds D-adenosine. Nature Biotechnolgy, 14, 1112-1115 (1996)

Nolte, A.; Klußmann, S.; Bald, R.; Erdmann, V.A.; Fürste, J.P.: Mirror-design of L-oligonucleotide ligands binding to L-arginine. Nature Biotechnology, 14, 1116-1119 (1996)

Bier, F.F. and Fürste, J.P., "Nucleic acid based sensors". In: Frontiers in biosensorics, Birkhäuser-Verlag, Basel, pp 96-120 (1997)

Kleinjung, F.; Klußmann, S.; Erdmann, V.A.; Scheller, F.W.; Fürste, J.P.; Bier, F.F.: High Affinity RNA as a Recognition Element in a Biosensor. Analytical Chemistry 70, 328-331 (1998)

Erdmann, V.A.; Fürste, J.-P.: Das „Netzwerk“ RNA-Technologien. euro-biotech 2000, 104-108 (1999)

Last update: 29.1.2001